Molecular analysis of carbohydrate-active enzymes and their applications

Faculty

Website

https://sites.google.com/elms.hokudai.ac.jp/molenzhpen/home

Selected Publications

1. Kumagai Y, Kishimura H, Lang W, Tagami T, Okuyama M, Kimura A.: Characterization of an unknown region linked to the glycoside hydrolase family 17 β-1,3-glucanase of Vibrio vulnificus reveals a novel glucan-binding domain. Drugs, 20, 250 (2022)
2. Lang W, Kumagai Y, Habu S, Sadahiro J, Tagami T, Okuyama M, Kitamura S, Sakairi S, Kimura A.: Physicochemical functionality of chimeric isomaltomegalosaccharides with α-(1→4)-glucosidic segments of various lengths. Polym., 291, 119562 (2022)
3. Tagami T, Chen M, Furunaga Y, Kikuchi A, Sadahiro J, Lang W, Okuyama M, Tanaka Y, Iwasaki T, Yao M, Kimura A.: Structural insights reveal the second base catalyst of isomaltose glucohydrolase. FEBS J., 289, 1118-1134 (2022)
4. Lang W, Kumagai Y, Sadahiro J, Saburi W, Sarnthima R, Tagami T, Okuyama M, Mori H, Sakairi N, Kim D, Kimura A.: A practical approach to producing isomaltomegalosaccharide using dextran dextrinase from Gluconobacter oxydans ATCC 11894. Appl. Microbiol. Biotechnol., 106, 689-698 (2022)
5. Okuyama M, Serizawa R, Tanuma M, Kikuchi A, Sadahiro J, Tagami T, Lang W, Kimura A.: Molecular insight into regioselectivity of transfructosylation catalyzed by GH68 levansucrase and β-fructofuranosidase. Biol. Chem., 296, 100398 (2021)

Specialized Fields

Food Sciences

Research Keywords

Carbohydrate-active Enzymes, Molecular Enzymology, Physiology of Glycosylases, Synthesis of Novel Carbohydrates