Biochemistry of functional proteins and application for carbohydrate bioengineering

Faculty

Website

http://www.agr.hokudai.ac.jp/biochem/

Selected Publications

1. Ryosuke Kawahara, Wataru Saburi, Rei Odaka, Hidenori Taguchi, Shigeaki Ito, Haruhide Mori, and Hirokazu Matsui, Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, β-1,4-mannooligosaccharide phosphorylase, The Journal of Biological Chemistry, 287, 42389-42399 (2012).
2. Ken Hamura, Wataru Saburi, Hirokazu Matsui, and Haruhide Mori, Modulation of acceptor specificity of Ruminococcus albus cellobiose phosphorylase through site-directed mutagenesis, Carbohydrate Research, 379, 21-25 (2013).
3. Tatsuya Sawano, Wataru Saburi, Ken Hamura, Hirokazu Matsui, and Haruhide Mori, Characterization of Ruminococcus albus cellodextrin phosphorylase and identification of a key phenylalanine residue for acceptor specificity and affinity to the phosphate group, FEBS Journal, 280, 4463-4473 (2013).
4. Takaaki Fujiwara, Wataru Saburi, Hirokazu Matsui, Haruhide Mori, and Min Yao, Structural insights into the epimerization of β-1,4-linked oligosaccharides catalyzed by cellobiose 2-epimerase, the sole enzyme epimerizaing non-anomeric hydroxyl groups of unmodified sugars, The Journal of Biological Chemistry, 289, 3405-3415 (2014).
5. Naoya Tamamura, Wataru Saburi, Atsushi Mukai, Naoki Morimoto, Toshihiro Takehana, Seiji Koike, Hirokazu Matsui, and Haruhide Mori, Enhancement of hydrolytic activity of thermophilic alkalophilic α-amylase from Bacillus AAH-31 through optimization of amino acid residues surrounding the substrate binding site, Biochemical Engineering Journal, 86, 8-15 (2014).

Specialized Fields

Research Keywords